- Protein Structure and Function
- Workflow Walkthrough
- Results Walkthrough
- Protein Design
- Workflow Walkthrough
Protein Structure and Function
Get information about protein sequences including identifying protein functional domains, predicting gene ontology and EC numbers, and predicting protein structure
- Navigate to the Protein Structure and Function launcher card. You can also find this workflow using the search tool at the top right corner or by using the “Protein Engineering” filter on the left-hand side
- Select version 1.0.2 from the dropdown menu in the top-right corner, and click “Run Workflow” when ready to begin analysis.
- Select the type of data you will input (FastA file, text sequence, or protein accession number), and upload the data. Then, select what type of protein sequence you have uploaded - monomer or multimer.
- Select the protein structure prediction algorithms to run (RaptorX, Rosettafold, or Alphafold) and whether to predict 2D structure only or both 2D and 3D structure.
- Give your workflow a unique name, and review workflow parameters and inputs. When satisfied and ready to run, click “Run Workflow”
- To view the results of your Protein Structure and Function workflow run, first find and open your corresponding workflow run in the Activity tab.
- Navigate to the Files tab. In the output folder, you can navigate, open, and download all workflow results files.
Predict new protein sequence with a similar structure to a known sequence
- Navigate to the Protein Design workflow on the Form Bio platform. You can locate this workflow by using the search bar in the top-right corner, or by using the Protein Engineering filter on the left hand side.
- Select version 1.0.0 from the dropdown menu in the top right corner. You may view information about the workflow run here, including use cases and a methods summary. When ready to begin, click Run Workflow.
- Upload a protein PDB file containing the protein sequence(s) of interest.
- Tune parameters of the workflow run, including number of redesigns to calculate, and which, if any, sections of the protein should remain constant.
- Give your workflow a unique name, and review workflow inputs and parameters. When ready to submit your run, click Run Workflow.