Gene Therapy

Gene Therapy

AAV PacBio Quality Control

Assess the completeness and contamination of PacBio sequenced adeno-associated virus (AAV) constructs by examining alignment coverage across sequences and among specific regions including the promoter and CDS.

Runtime Estimates

Average: 2 hours 18 minutes based on 15 test runs

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Workflow Walkthrough

Step 1: Navigate to the AAV PacBio Quality Control workflow on the Form Bio platform. You can use the Gene Therapy or Candidate Validation filters to help you locate the launcher.

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Step 2: Select version 1.0.6 from the dropdown tab in the top right corner.

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Step 3: Click “Run Workflow” in the top right corner.

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Step 4: Launcher Tabs

  1. Upload input data and select the type of sequences and whether Illumina data is included
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  3. Select AAV design and upload a transfer plasmid FastA sequence and a file describing genomic regions
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  5. Select reference genome and genome annotation version
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  7. Select the alignment algorithm and options for packaging/construct sequences
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  9. Review settings and name the workflow run. We will call this run “Test1”
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  11. Click Run Workflow in the bottom left corner to begin analysis