VALID
Verify Allogenic Loci in DNA
Workflow Walkthrough
- Navigate to the VALID workflow on the Form Bio platform. You can find the workflow using the search bar at the top right corner or by using the Genome Editing filter on the left-hand side.
- Select version 2.0.0 from the dropdown menu. When ready to begin, click Run Workflow.
- Select the type of sequencing to be analyzed, either Sanger or NGS. Then, select the type of editing experiment performed, and provide file inputs.
- Tune additional parameters related to the workflow run. For most purposes, default parameters are optimal.
- Give your workflow a unique name, and take a moment to review the chosen inputs and parameters. When ready to begin, click Run Workflow to submit your analysis.
Results Walkthrough
- To view the results of your VALID workflow run, first find and select your run from the Activity tab of the Form Bio platform.
- Once selected, press Open Analysis to view the results of your workflow run in a new tab. It might take a few moments to load the results viewer.
- View the results of your analysis on the new page that opens. Use the left-hand sidebar to navigate.
DR GENE
Design and Rank Guides for Editing Nucleotides with Enzymes
Run time estimate
Average = TBD
Workflow Walkthrough
- Navigate to DR GENE workflow on the Form Bio platform. You can search for this workflow using the bar at the top-right corner or by selecting the Genome Editing or Candidate Validation filters on the left-hand side.
- Select version 2.1.0 from the dropdown menu in the top right corner. Take a moment to review some information about the workflow analysis, inputs, and outputs. When ready to begin, click Run Workflow.
- Select one of three functions, either Genome Editing, Knock-Out, or Off-target search. Depending on your choice, you will be asked to tune certain parameters about the type of experiment to design guides for.
- Provide the necessary input file for your experiment. For Genome Editing this will be an edit table, for knock-out this will be a list of genes, and for off-target search this will be a spacer sequence list.
- For guide design functions, you’ll be asked to tune some parameters related to PCR primers. In most cases, defaults are desirable.
- For some genome editing algorithms, you’ll be asked to tune some additional parameters related to guide design for that specific algorithm.
- Give your workflow a unique name, and take a moment to review the chosen inputs and parameters. When ready to begin, click Run Workflow to submit your analysis.
Results Walkthrough
- Locate your workflow run from the Activity tab, and select it.
- On this page, you can view a variety of information about the workflow run, including inputs, outputs, and parameters. To view the analysis, click Open Analysis in the top right corner.
- A new tab will open containing sequences of interest for all selected editing experiments. Use the tabs in the top-left corner to navigate.
