- VALID
- Use Cases
- Summary and Methods
- Inputs
- Outputs
- Workflow Walkthrough
- Results Walkthrough
- Citations
- Built with
- DR GENE
- Use Cases
- Summary and Methods
- Inputs
- Outputs
- Workflow Walkthrough
- Results Walkthrough
- Citations
- Built with
VALID
Verify Allogenic Loci in DNA
Version 1.6.1
Use Cases
Evaluate the outcome of an editing experiment. For validating Sanger sequencing, please use our sangerAnalyzeR tool in FormBench.
Summary and Methods
This workflow is designed to help the user validate editing experiments. Currently, validation is supported for two sequencing types: Targeted Amplicon Sequencing and Whole Genome Sequencing. Click the toggles below to learn more about each of the supported sequencing types.
Targeted Amplicon Sequencing
Whole Genome Sequencing
Inputs
Outputs
Workflow Walkthrough
Results Walkthrough
Citations
Built with
DR GENE
Design and Rank Guides for Editing Nucleotides with Enzymes
Version 2.1.1
Use Cases
- The user wishes to predict and design optimal single-guide RNA (sgRNA) sequences for making a knock-out or edit in a genome.
- The user wishes to rank created guide sequences to determine efficacy for a particular experiment.
- The user wishes to determine potential off-target effects for created guide sequences.
Summary and Methods
This workflow is designed to help the user predict and design the best single-guide RNA (sgRNA) sequences for making a knock-out or edit in a genome. Currently, sgRNA design is supported for two functions: Knockout Experiment and Genome Editing Experiment. This is a new workflow based on improvements made to the CRISPRank and CRISPR Knock-out workflows with additional functionality and optimized algorithms. This workflow can also be used to perform off-target searches. Click the toggles below to learn more about each function.
Knockout Experiment
Genome Editing Experiment
Off-Target Search
Inputs
Outputs
Workflow Walkthrough
Results Walkthrough
Citations
Built with
