Quick Tools

Calculate Percent GC
Convert SnapGene DNA File
Detect CpG Islands
Download Gene Orthologs by Taxa Group
Download Gene Sequences
ePEG Design for Prime Editing
Find Masked Regions in Genome
gBLOCK Synthesis Tool
Generate Seq Files
Merge FastQ Sequence Files
Prime Edit Location Finder
Primer Design for PCR
SDS-PAGE Analysis
Sequence Length
Splits FastA Sequence Files
Splits FastQ Sequence Files
Transform Annotation File
Transform Sequence
Validate Bed and FastA Match

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Calculate Percent GC

Calculate fractional GC content of nucleic acid sequences.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Calculate Percent GC launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select whether you wish to input a file or text sequence. Upload the file or paste in the text sequence you wish to analyze.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Here, you can view input and output files, as well as a preview of the file and the fractional GC content of the sequence. You can choose to download the input, output, or both files.

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Convert SnapGene DNA File

Generate FastA and BED file from SnapGene DNA file.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Convert SnapGene DNA File launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Give your sequence a name - this will be used as the Sequence ID header for the FastA file.

Select your SnapGene DNA file by clicking the Select File button. This will open the file explorer, allowing you to search the directory of files.

Lastly, provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Detect CpG Islands

Calculate ratio of Gs and Cs over a window.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Detect CpG Islands launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To begin, select whether your input is a text sequence or a file. If a text sequence, you’ll be asked to paste your sequence in the provided box. If a file, you’ll be asked to upload the file from the file explorer.

You can optionally tune advanced parameters for the run, including the size of the window to search, and the minimum ratio of G/C observed and expected. Default settings will accomplish the needs of most users. Don’t forget to provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Download Gene Orthologs by Taxa Group

This workflow is designed to help the users download gene sequences from taxa group.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Download Gene Orthologs by Taxa Group launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

In the Gene space, provide the official gene symbol for the gene to download. In the Taxon Group space, select the taxon you wish to search.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Download Gene Sequences

This workflow is designed to help the users extract the gene sequences from a genome including the genomic, cds, and protein sequences.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Download Gene Sequences launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the type of data input source and the NCBI taxonomy ID number. Also provide a file containing gene symbols, one per line.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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ePEG Design for Prime Editing

Designs engineered pegRNA sequences for increased accuracy and editing in prime editing experiments.

Version 1.0.1

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Workflow Walkthrough

Navigate to the ePEG Design for Prime Editing launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Upload a file containing pegRNA information. This should a tab seperated file with the spacers, RT Templates, and PBS Sequences. Column names must be named and in the order: Spacer RTT PBS. This table can also be created within the workflow.Also provide the scaffold sequence and the length of the linker sequence to design. Select which motif sequence to use.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Find Masked Regions in Genome

Determine genome masked regions.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Find Masked Regions in Genome launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the DNA sequence file in FASTA format.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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gBLOCK Synthesis Tool

Splits a DNA sequence into synthesizable fragments for Gibson Cloning (HiFi Cloning).

Version 1.0.1

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Workflow Walkthrough

Navigate to the gBLOCK Synthesis Tool launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select whether you'd like to input text or select a file. Fill in the query with text if you select text or upload a file if you select a file. Determine the desired fragment length and overhang length.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Generate Seq Files

Generate FastA, Concat FastX, Convert Fq to FastA File; Interleave Fq.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Generate Seq Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select the type of transformation to be done from input file to output file. Also provide the FastA sequence file and the sequence ID for the sequence name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Merge FastQ Sequence Files

Generate large FastQ file from many FastQ files.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Merge FastQ Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the directory for the input files. Select which type of merge to perform and provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Prime Edit Location Finder

Determine the optimal location for large PASTE inserts.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Prime Edit Location Finder launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Enter both an insert sequence and a target sequence into which the insert will be inserted.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Primer Design for PCR

Designs pairs of primer sequences for PCR amplification using the primer3 tool.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Primer Design for PCR launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are providing your sequence as raw text or as a file. Select the file containing your sequence of interest or paste your sequence as raw text in the provided box.

On the next tab, you may also optionally tune advanced parameters pertaining to the types of primers you wish to design. The default sequences are sufficient for the needs of most users.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. Here you can view the designed primers. You can choose to download the input file, output file, or both.

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SDS-PAGE Analysis

This workflow can be used to analyze images from SDS-PAGE experiments.

Version 1.0.0

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Workflow Walkthrough

Navigate to the SDS-PAGE Analysis launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Choose a type of analysis to perform and upload the input image. Also choose the number of lanes and mark which one is the control lane.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Sequence Length

Calculate sequence length.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Sequence Length launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are uploading a file or a text input. Upload the file or paste in the text sequence of which you wish to measure the length. Provide an output file name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. The length of your query is provided in the preview.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Splits FastA Sequence Files

Generate FastA files from larger FastA file.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Splits FastA Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

First, provide the sequence file to be split. Then provide the number of sequences it should be split in to. Choose a validation method if desired, and whether or not to remove special characters from the sequence name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. All the split files are listed on the left.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Splits FastQ Sequence Files

Generate FastQ files from larger FastQ file

Version 1.0.1

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Workflow Walkthrough

Navigate to the Splits FastQ Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

First, provide the sequence file to be split. Then provide the number of sequences it should be split in to.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Transform Annotation File

Convert GFF/GTF Files

Version 1.0.1

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Workflow Walkthrough

Navigate to the Transform Annotation File launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Choose the type of transformation you want to be performed. Upload an input file and provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Transform Sequence

Translate DNA sequence to protein; reverse translate protein to DNA sequence; determine reverse complement DNA sequence

Version 1.0.1

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Workflow Walkthrough

Navigate to the Transform Sequence launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are providing your sequence as raw text or as a file. Select the file containing your sequence of interest or paste your sequence as raw text in the provided box. Choose which transformation to perform: translate DNA, reverse translate protein, or reverse complement.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Validate Bed and FastA Match

This workflow determines if a FastA file and a Bed file share any sequence ids.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Validate Bed and FastA Match launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide a sequence input file. Also upload (or create within the workflow) a genomic regions file (Bed file). Provide a name for the output files and chose which type of transformation to perform.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

Calculate Percent GC
Convert SnapGene DNA File
Detect CpG Islands
Download Gene Orthologs by Taxa Group
Download Gene Sequences
ePEG Design for Prime Editing
Find Masked Regions in Genome
gBLOCK Synthesis Tool
Generate Seq Files
Merge FastQ Sequence Files
Prime Edit Location Finder
Primer Design for PCR
SDS-PAGE Analysis
Sequence Length
Splits FastA Sequence Files
Splits FastQ Sequence Files
Transform Annotation File
Transform Sequence
Validate Bed and FastA Match

‣

Calculate Percent GC

Calculate fractional GC content of nucleic acid sequences.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Calculate Percent GC launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select whether you wish to input a file or text sequence. Upload the file or paste in the text sequence you wish to analyze.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Here, you can view input and output files, as well as a preview of the file and the fractional GC content of the sequence. You can choose to download the input, output, or both files.

‣

Convert SnapGene DNA File

Generate FastA and BED file from SnapGene DNA file.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Convert SnapGene DNA File launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Give your sequence a name - this will be used as the Sequence ID header for the FastA file.

Select your SnapGene DNA file by clicking the Select File button. This will open the file explorer, allowing you to search the directory of files.

Lastly, provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Detect CpG Islands

Calculate ratio of Gs and Cs over a window.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Detect CpG Islands launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To begin, select whether your input is a text sequence or a file. If a text sequence, you’ll be asked to paste your sequence in the provided box. If a file, you’ll be asked to upload the file from the file explorer.

You can optionally tune advanced parameters for the run, including the size of the window to search, and the minimum ratio of G/C observed and expected. Default settings will accomplish the needs of most users. Don’t forget to provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Download Gene Orthologs by Taxa Group

This workflow is designed to help the users download gene sequences from taxa group.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Download Gene Orthologs by Taxa Group launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

In the Gene space, provide the official gene symbol for the gene to download. In the Taxon Group space, select the taxon you wish to search.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Download Gene Sequences

This workflow is designed to help the users extract the gene sequences from a genome including the genomic, cds, and protein sequences.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Download Gene Sequences launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the type of data input source and the NCBI taxonomy ID number. Also provide a file containing gene symbols, one per line.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

‣

Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

ePEG Design for Prime Editing

Designs engineered pegRNA sequences for increased accuracy and editing in prime editing experiments.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the ePEG Design for Prime Editing launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Upload a file containing pegRNA information. This should a tab seperated file with the spacers, RT Templates, and PBS Sequences. Column names must be named and in the order: Spacer RTT PBS. This table can also be created within the workflow.Also provide the scaffold sequence and the length of the linker sequence to design. Select which motif sequence to use.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

‣

Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Find Masked Regions in Genome

Determine genome masked regions.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Find Masked Regions in Genome launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the DNA sequence file in FASTA format.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

‣

Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

gBLOCK Synthesis Tool

Splits a DNA sequence into synthesizable fragments for Gibson Cloning (HiFi Cloning).

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the gBLOCK Synthesis Tool launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select whether you'd like to input text or select a file. Fill in the query with text if you select text or upload a file if you select a file. Determine the desired fragment length and overhang length.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

‣

Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Generate Seq Files

Generate FastA, Concat FastX, Convert Fq to FastA File; Interleave Fq.

Version 1.0.1

‣

Workflow Walkthrough

Navigate to the Generate Seq Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Select the type of transformation to be done from input file to output file. Also provide the FastA sequence file and the sequence ID for the sequence name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

‣

Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

‣

Merge FastQ Sequence Files

Generate large FastQ file from many FastQ files.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Merge FastQ Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide the directory for the input files. Select which type of merge to perform and provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Prime Edit Location Finder

Determine the optimal location for large PASTE inserts.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Prime Edit Location Finder launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Enter both an insert sequence and a target sequence into which the insert will be inserted.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Primer Design for PCR

Designs pairs of primer sequences for PCR amplification using the primer3 tool.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Primer Design for PCR launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are providing your sequence as raw text or as a file. Select the file containing your sequence of interest or paste your sequence as raw text in the provided box.

On the next tab, you may also optionally tune advanced parameters pertaining to the types of primers you wish to design. The default sequences are sufficient for the needs of most users.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. Here you can view the designed primers. You can choose to download the input file, output file, or both.

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SDS-PAGE Analysis

This workflow can be used to analyze images from SDS-PAGE experiments.

Version 1.0.0

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Workflow Walkthrough

Navigate to the SDS-PAGE Analysis launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Choose a type of analysis to perform and upload the input image. Also choose the number of lanes and mark which one is the control lane.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.
Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Sequence Length

Calculate sequence length.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Sequence Length launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are uploading a file or a text input. Upload the file or paste in the text sequence of which you wish to measure the length. Provide an output file name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. The length of your query is provided in the preview.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Splits FastA Sequence Files

Generate FastA files from larger FastA file.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Splits FastA Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

First, provide the sequence file to be split. Then provide the number of sequences it should be split in to. Choose a validation method if desired, and whether or not to remove special characters from the sequence name.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab. All the split files are listed on the left.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Splits FastQ Sequence Files

Generate FastQ files from larger FastQ file

Version 1.0.1

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Workflow Walkthrough

Navigate to the Splits FastQ Sequence Files launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

First, provide the sequence file to be split. Then provide the number of sequences it should be split in to.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Transform Annotation File

Convert GFF/GTF Files

Version 1.0.1

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Workflow Walkthrough

Navigate to the Transform Annotation File launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Choose the type of transformation you want to be performed. Upload an input file and provide a name for the output file.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Transform Sequence

Translate DNA sequence to protein; reverse translate protein to DNA sequence; determine reverse complement DNA sequence

Version 1.0.1

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Workflow Walkthrough

Navigate to the Transform Sequence launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

To start, select whether you are providing your sequence as raw text or as a file. Select the file containing your sequence of interest or paste your sequence as raw text in the provided box. Choose which transformation to perform: translate DNA, reverse translate protein, or reverse complement.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.

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Validate Bed and FastA Match

This workflow determines if a FastA file and a Bed file share any sequence ids.

Version 1.0.1

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Workflow Walkthrough

Navigate to the Validate Bed and FastA Match launcher card. You can use the search bar at the top right corner, or use the Quick Tool tag to find the workflow card.
Select the version from the dropdown menu in the top right corner. When ready to begin, click “Run Workflow”.

Provide a sequence input file. Also upload (or create within the workflow) a genomic regions file (Bed file). Provide a name for the output files and chose which type of transformation to perform.

On the next tab, give your workflow run a unique name, and review the input data and run parameters. When ready to submit, click “Run Workflow”.

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Results Walkthrough

Find your completed workflow run from the Activity tab of the platform. You can use the search bar to search for it. Select your workflow run for more information.
Upon selection, results from your workflow run are summarized in the Results tab.

Navigate to the All Files tab to view and download analysis outputs in the output folder. These folders are also available in the File Explorer.