AUC (% Partials)

AUC (% Partials) or Full-Partial Assay

Determining the proportions of full and partial (partially full) capsids is a crucial step in evaluating vector designs for gene therapy. Typically, these evaluations are carried out in the lab after a small scale viral production run and assayed with methods including analytical ultracentrifugation (AUC), mass photometry (MP), transmission electron microscopy (TEM), or most recently, long-read sequencing.

We have tested several vector designs with the full-partial in silico assay and produced the virus for characterization with AUC or MP and long-read sequencing.

The figure shows the encapsidated full and partial results from the lab AUC assay (left) and  full-partial in silico assay (right). We find that the AUC lab assay is well-represented by the full-partial in silico assay results and produces highly comparable sedimentation curves with similar proportions for full and partial genomes.

For further details, please see ISA example.

Group sedimentation coefficients. Encapsidated full and partial results from the lab AUC assay (left panel) and genome counts by sedimentation coefficients from the full-partial in silico assay (right panel); N=4. Vector p5 (blue).